Methylation analysis of Igf2 / H19 Imprinting Control Region (ICR) in Type II Diabetes Mellitus induced mice using Methylation-Specific PCR

The imprinting control region (ICR) is a that controls imprinted expression of Igf2/H19 so Igf2 will only be expressed from the paternal allele instead maternal allele. This research aimed to reveal if ICR methylation status' change contributes emergence type II diabetes Mellitus (T2DM) throughout induction in mice model. changes were detected by methylation-specific PCR (MSP). Control group was given normal diet and buffer vehicle injection while treatment high fat (HFD) six times 60 mg/kg body weight streptozotocin injection. process conducted for 12 weeks. insulin tolerance test (ITT) on week 0, 9, 12. treatment's successfully induced significantly higher area under curve (AUC) compared (P < 05). Moreover, ITT showed diabetic had glucose levels at every time point test. Meanwhile, there no evident through 0 Thus, not proven contribute T2DM.